Hiv Protocols

Item Information
Item#: 9781493930456
Edition 03
Author Prasad & Kalpana (Eds)
Cover Hardback
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This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and Neuro AIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successfulMethods in Molecular Biologyseries format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,HIV Protocols, Third Editionis a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.

Table of Contents
Part I HIV-1 Replication - Early Events1. Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System<
>Nicholas E. Webb and Benhur Lee2. Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry<
>Natasha D. Durham and Benjamin K. Chen3. HIV-1 Capsid Stabilization Assay<
>Thomas Fricke and Felipe Diaz-Griffero4. Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity<
>Joao I. Mamede and Thomas J. Hope5. HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations<
>Joseph A. Hollenbaugh and Baek Kim6. Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector<
>Jonathan M.O. Rawson, Christine L. Clouser, and Louis M. ManskyPart II HIV-1 RNA Structure and RNA-Protein Interactions 7. Novel Biochemical Tools for Probing HIV RNA Structure<
>Jason W. Rausch, Joanna Sztuba-Solinska, Sa
ina Lusvarghi, and Stuart F. J. Le Grice8. Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq<
>Sebla B. Kutluay and Paul D. Bieniasz9. Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations<
>Deepali Singh, Ioana Boeras, Gatikrushna Singh, and Kathleen Boris-LawriePart III HIV-1 Replication - Post-Integrational Events 10. Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase<
>Jacques J. Kessl, Amit Sharma, and Mamuka Kvaratskhelia11. Quantification of HIV-1 Gag Localization Within Virus Producer Cells<
>Annalena La Porte and Ganjam V. Kalpana12. Methods to Study Determinants for Mem
ane Targeting of HIV-1 GagIn Vitro<
>Ga
ielle C. Todd and Akira OnoPart IV Studying HIV-1 Replication and Pathogenesis in Animal Models 13. Visualizing the Behavior of HIV-Infected T CellsIn VivoUsing Multiphoton Intravital Microscopy<
>Radwa Sharaf, Thorsten R. Mempel, and Thomas T. Murooka14. Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice<
>Milena Veselinovic, Paige Charlins, and Ramesh Akkina15. High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis<
>Tynisha Thomas, Kieran Seay,Jian Hua Zheng, Cong Zhang,Christina Ochsenbauer, John C. Kappes,and Harris GoldsteinPart V Tools to Study HIV-1 Latency and Pathogenesis 16. Measuring the Frequency of Latent HIV-1 in Resting CD4+T Cells Using a Limiting Dilution Co-Culture Assay<
>Gregory M. Laird, Daniel I.S. Rosenbloom, Jun Lai, Robert F. Siliciano, and Janet D. Siliciano17. LGITIn VitroLatency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds<
>Ulrike Jung, Mayumi Takahashi, John J. Rossi, and John C. Burnett18. Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies<
>Eliseo A. Eugenin and Joan W. Berman19. Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells<
>Nigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, and Michael Bukrinsky 20. The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients<
>Nicole A. Haverland, Lance M. Villeneuve, Pawel Ciborowski, and Howard S. Fox21. Proteomic Characterization of Exosomes from HIV-1-Infected Cells<
>Ming Li and Bharat RamratnamPart VI NeuroAIDS 22. Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot<
>Fabienne Rayne, Solène Debaisieux, Annie Tu, Christophe Chopard, Petra Tryoen, and Bruno Beaumelle23. Protocol for Detection of HIV-Tat Protein in Cere
ospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay<
>Tory P. Johnson and Avindra Nath24. Measuring the Uptake and Transactivation Function ofHIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up<
>Arthur P. Ruiz and Vinayaka R. Prasad25. Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures<
>Vasudev R. Rao, Eliseo A. Eugenin, and Vinayaka R. Prasad